Way before Z-280.
Like at Z-222 as indicated by his lapel flap at Z-224 and the bulging out of his jacket the frame before that.
Or do you think those things were caused by a gust of wind?
I don't see a bulge on JBC's chest. How does the jacket bulge from a bullet but not the shirt? And we can see that the shirt doesn't move because if it did the tie would move. It doesn't budge:
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What we see is a change in the amount of visible white shirt. That can be due to the jacket moving because his right arm is moving as we see happening in subsequent frames.
JBC is definitely reacting to the shot that struck JFK in the neck. But, as Dan has shown, that was the first shot. So either JBC and Nellie were very mistaken that JBC was hit in the back by the second shot, or JBC was not yet hit in the back then.